Overview
PMI Technology

PMI Technology

PMI technology enables the capture of known or unknown proteins starting only with gene sequence or protein sequence information. A synthetic peptide corresponding to the known or predicted partial sequence of a protein is “imprinted” on the surface of a polymer matrix. The imprints on the polymer, in the form of films, arrays, or beads have a high affinity and specificity for the target protein.

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Phase 1: Peptide Synthesis

A peptide is synthesized that corresponds to a signature sequence of a protein. The sequence may be experimentally derived or may be drawn from a database of predicted sequences.

Phase 2: Self Assembly

A universal matrix of polymerizable monomers is allowed to self assemble around the peptide and crosslinked into place.

Phase 3: Extraction

The peptide, or template, is then removed leaving behind a cavity complementary in shape and functionality. The cavities can be formed on a film, discrete sites of an array or the surface of beads.

Phase 4: Capture

When a sample of denatured proteins is exposed to the capture agent, the polymer will selectively retain the target protein and exclude all others.

Phase 5: Detection

After the washing, only the bound proteins remain. Common staining and tagging procedures can be used to detect expression levels and post translational modifications. Alternatively, the captured proteins can be eluted.